机能实验报告
Ach对离体家兔小肠运动作用
摘要
目的：观察乙酰胆碱对离体家兔小肠肠肌的影响和机制。方法：将离体小肠固定在离体小肠灌流装置里，在保证各影响因素不相互干扰的情况下,分别给予家兔离体肠肌标本生理盐水（Nacl）、Ach、阿托品、阿托品+乙酰胆碱刺激，完成每次刺激并出现明显现象后用38℃的台氏液冲洗肠肌标本，观察其收缩活动的特点并记录。结果：结果显示当滴加生理盐水（Nacl）刺激离体肠肌时，张力曲线没什么变化；当用乙酰胆碱刺激离体肠肌时，张力曲线则明显上升，且频率加快；当滴加阿托品刺激时，张力曲线明显下降，且频率减慢；当滴加乙酰胆碱加上阿托品刺激时，张力曲线变化较不规则。结论：乙酰胆碱（Ach）对离体家兔肠肌有增强其运动的作用，且作用于M受体。 中文关键词：离体家兔小肠肠肌；乙酰胆碱；张力曲线 Abstract：
Objective: to observe the acetylcholine on the influence and mechanism of intestinal muscle of rabbit small intestine in vitro. Methods: in vitro small intestine in vitro intestinal perfusion device fixed, in guarantee under the condition of each influence factor does not interfere with each other, in vitro intestinal muscle specimens were given the rabbit saline (Nacl), Ach, atropine, atropine + acetylcholine stimulation, complete with 38 ℃ after each stimulus and a significant phenomenon of Chinese Taiwan's fluid colonics muscle specimens and observe the chara...