摘要：研究采用HPLC法对5个不同品种的柴胡（Bupleurum chinense DC.）根样品进行了柴胡皂苷a、柴胡皂苷d的定量分析。结果表明，不同品种中柴胡皂苷a、柴胡皂苷d含量差异较大，这可能与柴胡的生长环境、采收年限等因素的影响有关。与传统方法相比较，HPLC法不仅操作简便、快捷，而且分析方法准确，重现性好。
关键词：柴胡（Bupleurum chinense DC.）；HPLC；柴胡皂苷a；柴胡皂苷d
中图分类号：； 文献标识码：A 文章编号：0439-8114（2017）13-2519-02
DOI：
The HPLC Method for Determination of Saikosaponin a and d Content in Bupleurum from Different Habitats
MA Ze-gang， HUANG Chun-hua， ZHONG Hui-yun
（Department of Pharmacy， Sichuan Vocational College of Health and Rehabilitation， Zigong 643000， Sichuan，China）
Abstract： The HPLC method for quantitative determination of saikosaponin a and saikosaponin d content in 5 Bupleurum samples from different habitats was applied. The results showed that， the saikosaponin a and d content of different varieties varied greatly， the reason of it may be related to the growing environment， harvesting years and other factors. Compared with traditional methods， the HPLC was a simple， quick， accurate m...